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  2. 小型蛋白垂直電泳槽

    伯樂Mini-Protean Tetra蛋白電泳槽

    伯樂Mini-Protean Tetra小型蛋白電泳槽可容納1~4塊小型手灌膠或Ready Gel 預制膠工作,用于蛋白SDS-PAGE垂直電泳分離。本欄目提供Mini-Protean Tetra的組成及選型配置方案。

    設備名稱:蛋白垂直電泳槽  蛋白垂直電泳儀
    制 造 商:美國Bio-Rad公司           產 地:新加坡/中國
    品    牌:伯樂(Bio-Rad)    
    型    號:Mini-Protean Tetra
    電泳配件:見[主要附件]  商品價格:見[訂貨指南]

    主要功能用途

    Bio-Rad Mini- Protean Tetra蛋白垂直電泳槽,是Western Blot實驗流程重要組成部件,可容納1~4 塊小型手灌膠或Ready Gel 預制膠(10×8.3cm)的電泳,主要用于蛋白聚丙烯酰胺凝膠電泳(PAGE或SDS-PAGE)實驗。

     

    性能特點

    伯樂Mini-Protean Tetra蛋白垂直電泳槽,兼容Mini-Protean precast gels預制凝膠、Ready Gel precast gels預制凝膠或手工自制PAGE凝膠。

    可在一小時內完成多達四塊小型蛋白凝膠的電泳分離。

    軟橡膠墊片與玻璃板形成嚴密密封,確保無泄漏灌膠。

    帶有簡單凸輪的灌制框,確保在任何平面上實現精確對齊。

    平行排列的灌膠架,能同時灌制兩塊凝膠,彈簧杠桿設計使得軟橡膠沉淀產生良好的密封性。

    封邊墊條的長玻板加厚,不易破碎。

    標有厚度和孔數的玻板和加樣梳便于識別。

    上樣向導防止泳道遺漏上樣或重復上樣。

    可互換模塊可將Mini- Protean Tetra電泳槽轉換為可進行Western轉印的 Mini Trans-Blot轉印槽。

     

     

    主要技術規格

    伯樂Mini-Protean Tetra蛋白垂直電泳槽適用的凝膠類型包括:Mini- Protean預制膠、手灌膠

    凝膠數:1–4塊

    凝膠格式:小型

    適用的凝膠尺寸(寬×長)

    預制膠:8.6×6.8cm;

    手灌膠:8.3×7.3cm

    玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm

    緩沖液運行需求

    運行2塊凝膠緩沖液總體積:700mL

    運行4塊凝膠緩沖液總體積:1000 mL

    SDS-PAGE 典型運行時間:35–45 分鐘(在200V恒定電壓下)

    Mini-PROTEAN Tetra加樣梳每孔最大上樣量

    1.0mm 10孔梳:44 μL

    1.0mm 15孔梳:26 μL

     

    安裝運行條件

    相對濕度范圍:0~95% R.H.

    工作環境溫度:0℃~40℃

    外觀尺寸(寬×長×高):12×16×18cm

    Mini- Protean Tetra 電泳槽重量:1.0kg

    (電泳電源另配)

     

    組成配置

    電泳槽系統

    伯樂Mini-Protean Tetra蛋白電泳槽全套工作裝置包括電泳緩沖液槽、電極組件、制膠組件輔助工具耗材。
    按一次可運行的最大凝膠數量,分為4-gel、2-gel兩種規格。
    如需跑4塊凝膠,可訂購4凝膠槽,也可在2凝膠套裝基礎上加配Mini-Protean Tetra相應功能附件,擴展為4塊凝膠工作通量。

    此外,根據使用的凝膠來源類型,還可單獨配置伯樂預制膠專用套裝。

    品名

    品牌

    貨號

    規格

    描述

    報價

    蛋白垂直電泳槽

    (4-gel/10-well/1.0mm)

    Bio-Rad

    1658001

    1套

    Mini-PROTEAN Tetra Cell

    4-gel system, 1.0mm gel thickness

    includes:

    Tank, lid with power cables

    Mini cell buffer dam;

    1×electrode assembly;

    1×companion running module(1658038);

    2×casting stands, 4 casting   frames;

    5 sets of short plates and spacer plates;

    5×10-well 1.0mm combs

    5×gel releasers(1653320)

    詢價

    詢價

    蛋白垂直電泳槽

    (4-gel/10-well/1.5mm)

    Bio-Rad

    1658006

    1套

    Mini-PROTEAN Tetra Cell

    4-gel system, 1.5mm gel thickness

    includes:

    Tank, lid, power cables

    Mini cell buffer dam;

    1×electrode assembly;

    1×companion running module(1658038);

    2×casting stands, 4 casting   frames;

    5 sets of short plates and spacer plates;

    5×10-well 1.5mm combs

    5×gel releasers(1653320)

    詢價

    詢價

    蛋白垂直電泳槽

    (4-gel/10-well/0.75mm)

    Bio-Rad

    1658000

    1套

    Mini-PROTEAN Tetra Cell

    4-gel system, 0.75mm gel thickness

    includes:

    Tank, lid, power cables

    Mini cell buffer dam;

    1×electrode assembly;

    1×companion running module(1658038);

    2×casting stands, 4 casting   frames;

    5 sets of short plates and spacer plates;

    5×10-well 0.75mm combs

    5×gel releasers(1653320)

    詢價

    詢價

    蛋白垂直電泳槽l

    (2-gel/10-well/1.0mm)

    Bio-Rad

    1658003

    1套

    Mini-PROTEAN Tetra Cell

    2-gel system, 1.0mm gel thickness

    includes:

    1×casting stand, 2×casting frames;

    5×10-well combs;

    5 short plates and 5 spacer plates

    詢價

    詢價

    蛋白垂直電泳槽

    (2-gel/10-well/1.5mm)

    Bio-Rad

    1658007

    1套

    Mini-PROTEAN Tetra Cell

    2-gel system, 1.5mm gel thickness

    includes:

    1×casting stand, 2×casting frames;

    5×10-well combs;

    5 sets of glass plates

    詢價

    詢價

    蛋白垂直電泳槽

    (2-gel/10-well/0.75mm)

    Bio-Rad

    1658002

    1套

    Mini-PROTEAN Tetra Cell

    2-gel system, 0.75 mm gel thickness

    includes:

    1×casting stand, 2×casting   frames;

    5×10-well combs;

    5 short plates and 5 spacer plates

    詢價

    詢價

    Mini Precast Gels(4-gel)

    預制膠蛋白電泳槽

    Bio-Rad

    1658004

    1套

    Mini-PROTEAN Tetra Vertical Electrophoresis Cell

    for Mini Precast Gels, 4-gel

    includes:

    electrode assembly;

    companion running module;

    tank, lid with power cables, cell buffer   dam

    詢價

    詢價

    Mini Precast Gels (2-gel)

    預制膠蛋白電泳槽

    Bio-Rad

    1658005

    1套

    Mini-PROTEAN Tetra Vertical Electrophoresis  Cell

    for Mini Precast Gels, 2-gel

    includes:

    electrode assembly;

    tank, lid with power cables, cell buffer dam

    詢價

    詢價



    電泳槽組件

    伯樂Mini-Protean Tetra 小型蛋白垂直電泳槽的制膠模塊(Casting Module),是指用于灌制特定厚薄和齒孔數目蛋白凝膠所需的模塊化套裝。

    模塊由通用核心組件(Core System)和差異化功能組件組成。通用核心灌膠組件為灌膠架(含4個密封墊),夾膠框(casting frames)和或膠鏟(gel releaser)。差異化功能組件則包括特定規格的玻璃板套件,電泳梳和上樣導槽。

    伯樂Mini-PROTEAN Tetra Cell小型垂直電泳槽有多種灌膠模塊可選,分別滿足不同上樣量和對凝膠厚度的特殊要求。

    品名

    品牌

    貨號

    規格

    描述

    報價

    1.0mm/10孔 制膠模塊

    Bio-Rad

    1658015

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    10-well/1.0 mm gel casting module

    includes:

    2×casting stands; 4×casting   frames;

    5×combs, 5 short plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    1.0mm/15孔制膠模塊

    Bio-Rad

    1658016

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    15-well, 1.0 mm gel casting module

    includes:

    2×casting stands; 4×casting   frames;

    5×combs, 5 short   plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    1.0mm/5孔 制膠模塊

    Bio-Rad

    1658013

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    5-well,   1.0 mm gel casting module

    includes:

    2×casting stands; 4×casting   frames;

    5×combs, 5 short   plates and 5 spacer plates.

    詢價

    詢價

    1.0mm/9孔 制膠模塊

    Bio-Rad

    1658014

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    9-well,   1.0 mm gel casting module

    includes:

    2×casting stands; 4×casting   frames;

    5×combs, 5 short plates and 5 spacer plates;

    1×sample loading guide.

    詢價

    詢價

    1.5mm/10孔 制膠模塊

    Bio-Rad

    1658021

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    10-well, 1.5 mm gel casting module

    includes:

    2×casting stands; 4×casting   frames;

    5×combs, 5 short plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    1.5mm/15孔 制膠模塊

    Bio-Rad

    1658022

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    15-well, 1.5 mm gel casting module

    includes:

    2×casting stands, 4×casting frames;

    5×combs, 5 short   plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    1.5mm/5孔 制膠模塊

    Bio-Rad

    1658019

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    5-well,   1.5 mm gel casting module

    includes   the following componets:

    2   casting stands, 4 casting frames

    5   combs, 5 short plates and 5 spacer plates.

    詢價

    詢價

    1.5mm/9孔 制膠模塊

    Bio-Rad

    1658020

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    9-well,   1.5 mm gel casting module

    includes:

    2×casting stands, 4×casting   frames;

    5×combs, 5 short   plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    0.75mm/10孔 制膠模塊

    Bio-Rad

    1658010

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    10-well,   0.75 mm gel casting module

    includes   the following componets:

    2   casting stands, 4 casting frames

    5   combs, 5 short plates and 5 spacer plates

    sample   loading guide.

    詢價

    詢價

    0.75mm/15孔 制膠模塊

    Bio-Rad

    1658011

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    15-well,   0.75 mm gel casting module

    includes:

    2×casting stands, 4×casting   frames;

    5×combs, 5 short plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    0.75mm/5孔 制膠模塊

    Bio-Rad

    1658008

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    5-well,   0.75 mm gel casting module

    includes:

    2×casting stands, 4×casting   frames;

    5×combs, 5 short   plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    0.75mm/9孔 制膠模塊

    Bio-Rad

    1658009

    1套

    Mini-PROTEAN Tetra Cell Casting Module

    9-well,   0.75 mm gel casting module

    includes:

    2×casting stands, 4×casting   frames;

    5×combs, 5 short plates and 5 spacer plates;

    1×sample loading guide

    詢價

    詢價

    灌膠通用套件(4膠)

    Bio-Rad

    1658051

    1套

    Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

    includes:

    2×stands, 4×frames,4×gaskets;

    5×gel   releasers.

    適用于4膠Mini-Protean Tetra電泳槽

    詢價

    詢價

    灌膠通用套件(2膠)

    Bio-Rad

    1658052

    1套

    Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

    includes:

    1×stand, 2×frames, 2×gaskets;

    5×gel releasers.

    適用于2膠Mini-Protean Tetra電泳槽

    詢價

    詢價

    基本耗材

    伯樂Mini-Protean Tetra垂直電泳槽工作的基本耗材主要包括蛋白凝膠玻璃板等。

    品名

    品牌

    貨號

    規格

    描述

    報價

    1.0mm厚玻璃板

    Bio-Rad

    1653311

    5塊/包

    Spacer Plates with 1.0 mm Integrated Spacers

    帶1.0mm厚封邊條,為1.0mm凝膠灌膠組件。

    詢價

    1.5mm厚玻璃板

    Bio-Rad

    1653312

    5塊/包

    spacer plate   with 1.5 mm integrated spacer

    帶1.5mm厚封邊條,用于1.5mm凝膠的制備

    詢價

    薄玻璃板

    Bio-Rad

    1653308

    5塊/包

    short plate for Mini-PROTEAN Tetra System

     and Mini-PROTEAN 3 Multi-Casting Chamber.

    外形尺寸(W×L):101× 73 mm

    與帶任一厚度封邊墊條的長玻璃板配套使用

    詢價

     

    訂貨指南

    1、蛋白電泳槽的選型

    因不同實驗樣品樣品數量、蛋白類型和表達豐度、實驗經驗與操作習慣的不同,選擇蛋白電泳槽重點要結合所用凝膠的厚度(0.75mm、1.0mm、1.5mm)、每張凝膠電泳的上樣孔數(常用的10孔-最大上樣量66μl、15孔-40μl)、每輪電泳的凝膠通量(4膠、2膠)和凝膠類型(手灌膠、預制膠)來確定具體選型方案。

    如單獨訂購Mini-Protean Tetra小型蛋白垂直電泳槽或更換電泳灌膠配件,請請參考本欄目“組成配置”“主機”部分所列各型電泳槽的技術規格和詳細配置內容。最常用的1658001槽單元,其標準配置適用的是1.0mm/10-well/4-gel凝膠。

     

    2、蛋白電泳槽電源的選擇

    伯樂Mini-Protean Tetra小型蛋白垂直電泳槽本身工作運行對電源并無特殊要求。伯樂PowerPac Basic 、PowerPac HC、PowerPac Universal等多個型號均可滿足同時給1-4個電泳槽供電的要求。

    但須注意的是,通常情況下,蛋白電泳槽只是Western Blot實驗流程的一個功能組件,單個電泳槽輸出凝膠數量(2塊或4塊)與電轉印槽(2塊)各自最大凝膠工作通量不同,要充分考慮實驗流程上的有效銜接問題。

    特別是當實驗樣品數量多于4塊凝膠的情況下,根據自身實驗條件和經驗,通盤考慮電轉印槽、電轉印模塊的配置數量。

    與常規140V左右恒壓蛋白電泳跑膠不同,電轉印對電流要求較高。通常,每塊凝膠-印跡膜的工作電流可達200mA甚至更高。若同時進行4塊凝膠轉膜操作,單一Basic電源很可能無法滿足供電要求。故需考慮HC、PowerPac Universal等具有電流輸出能力大于0.8A的電源型號確有必要。

     

    3、電泳槽與與Mini-Trans Blot小型蛋白電泳轉印槽搭配

    電轉移槽的選型配置問題,請參考Mini-Trans Blot小型蛋白轉印槽欄目內容。


    序號

    品名

    貨號

    套裝配置描述

    報價

    1-1

    Basic蛋白電泳套裝

    (1.0mm/10-well/4-gel)

    1658025

    Mini-PROTEAN Tetra and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra 4-gel system (1658001);

    PowerPac Basic power supply (1645050).

    詢價

    詢價

    1-2

    HC蛋白電泳套裝

    (1.0mm/10-well/4-gel)

    1658027

    Mini-PROTEAN Tetra and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra 4-gel system (1658001);

    PowerPac HC power supply (1645052).

    詢價

    詢價






    2-1

    電泳-轉印槽組合裝

    (1.0mm/10-well/4-gel/2-blot)

    1658029

    Electrophoresis and blot Bundle (10-well/1.0mm/4-gel)

    includes:

    Mini-PROTEAN Tetra Cell system (1658001);

    Mini Trans-Blot Module (1703935).

    詢價

    詢價

    2-2

    Basic電源-電泳-轉印套裝

    (1.0mm/10-well/4-gel/2-blot)

    1658033

    Electrophoresis, blot and Power Supply   Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價

    2-3

    HC電源-電泳-轉印套裝

    (1.0mm/10-well/4-gel/2-blot)

    1658035

    Electrophoresis, blot and Power Supply Bundle

    for 10-well, 1.0 mm, 4-gel system

    includes:

    Mini-PROTEAN Tetra Cell (1658001);

    Mini Trans-Blot Module (1703935);

    PowerPac HC Power Supply (1645052)

    詢價

    詢價

    2-4

    HC電源-電泳-轉印套裝

    (1.0mm/10-well/4-gel/4-blot)

    1658035-S

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

    Mini Trans-Blot Transfer Cell (1703930);

    Mini Trans-Blot Module (1703935);

    PowerPac HC Power Supply (1645052).

    詢價

    詢價

    2-5

    HC電源-電泳-轉印高級套裝

    (1.0mm/10-well/4-gel)

    (1.5mm/10-well/4-gel)

    (4-blot)

    1658035-U

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

    Mini-PROTEAN Tetra Cell Casting Module (1658021);

    Mini Trans-Blot Cell (1703930);

    Mini Trans-Blot Module (1703935);

    PowerPac HC Power Supply (1645052).

    詢價

    詢價






    3-1

    電泳-轉印槽組合裝

    (1.5mm/10-well/2-gel/2-blot)

    16580007-B

    Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

    includes:

    Mini-PROTEAN   Tetra Cell for handcast 2-gel (1658007);

    Mini Trans-Blot Module (1703935).

    詢價

    詢價

    3-2

    電泳-轉印組合裝

    (1.5mm/10-well/4-gel/2-blot)

    16580006-B

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

    Mini Trans-Blot Module (1703935);

    詢價

    詢價

    3-3

    Basic電源-電泳-轉印套裝

    (1.5mm/10-well/2-gel/2-blot)

    16580007-S

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價

    3-4

    Basic電源-電泳-轉印套裝

    (1.5mm/10-well/4-gel/2-blot)

    16580006-S

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEA Tetra Cell for handcast 4-gel (1658006);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價

    3-5

    HC電源-電泳-轉印套裝

    (1.5mm/10-well/4-gel/4-blot)

    16580006-U

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

    Mini Trans-Blot Module (1703935);

    Mini Trans-Blot Cell (1703930)

    PowerPac HC Power Supply (1645052).

    詢價

    詢價






    4-1

    電泳-轉印槽組合裝

    (1.0mm/10-well/2-gel)

    1658003-C

    Electrophoresis and blot Bundle (10-well/1.0mm/2-gel)

    includes:

    Mini-PROTEAN Tetra Cell system (1658003);

    Mini Trans-Blot Module (1703935).

    詢價

    詢價

    4-2

    Basic電源-電泳-轉印套裝

    (1.0mm/10-well/2-gel/2-blot)

    1658003-S

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價

    4-3

    電泳-轉印槽組合裝

    (1.5mm/10-well/2-gel)

    1658007-C

    Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

    includes:

    Mini-PROTEAN Tetra Cell system (1658007);

    Mini Trans-Blot Module (1703935).

    詢價

    詢價

    4-4

    Basic電源-電泳-轉印套裝

    (1.5mm/10-well/2-gel/2-blot)

    1658007-S

    Electrophoresis, blot and Power Supply Bundle

    includes:

    Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價






    5-1

    蛋白預制膠

    電泳-轉印槽組合

    1658030

    Vertical Electrophoresis and Mini Trans-Blot Module Bundle

    for Mini Precast 4-Gel

    includes:

    4-gel Mini-PROTEAN Vertical Cell (1658004);

    Mini Trans-Blot Module (1703935).

    詢價

    詢價

    5-2

    蛋白預制膠

    Basic電源電泳轉印套裝

    1658034

    Electrophoresis, blot and Power Supply Bundle

    for precast 4-gel

    includes:

    Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004);

    Mini Trans-Blot Module (1703935);

    PowerPac Basic Power Supply (1645050).

    詢價

    詢價

    5-3

    蛋白預制膠

    HC電源電泳轉印套裝

    1658036

    Electrophoresis, blot and Power Supply Bundle

    includes:

    for Mini Precast Gels, 4-gel

    Mini-PROTEAN Tetra Cell (1658004);

    Mini Trans-Blot Module (1703935);

    PowerPac HC Power Supply (1645052).

    詢價

    詢價

    (更多配置方案詳情請咨詢客服)

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